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β-Barrel Protein Design

With the availability of profound protein design and engineering knowledge, certified technical staff, and numerous state-of-the-art instruments, Creative Biolabs has developed advanced β-barrel protein design solutions for worldwide customers. With our proven competencies and regulatory expertise, we are therefore confident in offering the best services for your projects.

Introduction to β-Barrel Proteins

β-barrel proteins, also known as outer membrane proteins (OMPs), play a significant role in regulating signal transduction and membrane formation. Previous studies have shown that a number of strands, such as NspA, OmpA, OmpX, PagP, EstA, LpxR, as well as NalP, have been observed in all OMPs. The results suggest these strands are essential to a wide variety of biological functions, including acting as enzymes, transporters. Meanwhile, the structure of β-barrel proteins has been identified in recent years. For example, EstA has been considered as an attractive tool for mediating cell motility and surface protein presentation. Furthermore, many advances have been made on the crystallization of β-barrel proteins in native-like lipid environments. A series of assays, such as bicelle crystallization kit, lipidic cubic phase (LCP), have been developed for screening crystallization at a fast turnaround time. Additionally, the structure analysis of target proteins has led to more novel β-barrels in protein design and protein engineering for disease therapy.

The β-Barrel Protein Design. Fig.1 The β-Barrel Protein Design.

Services

The structure analysis of β-barrel proteins has aroused much attention and has been widely used for new β-barrel protein design and protein engineering. A panel of crystallization and sample preparation techniques have been established to predict the structure of β-barrel proteins. As a consequence, Creative Biolabs provides a range of services on analyzing the structure of current β-barrel to design novel β-barrel proteins for disease treatments. Up to now, we have developed several methods to predict their 3D structures, test their functions, as well as determine homogeneous samples. For instance, recent reports conducted by our labs have demonstrated that our methods can be broadly used to engineer protein nanopores by using β-barrel proteins. These methods can help our clients understand the organizing principle of β-barrel proteins. Besides, we offer a unique platform to design new structures of β-barrels for our worldwide customers to meet their requirements on drug delivery for disease therapy.

Folding pathways for β-barrel proteins in Gram-negative bacteria, mitochondria, and chloroplasts. Fig.2 Folding pathways for β-barrel proteins in Gram-negative bacteria, mitochondria, and chloroplasts. (Fairman, 2011)

As a leading service provider in the field of protein design and construction, Creative Biolabs is pleased to share our cutting-edge technology and extensive expertise in the field of protein studies with our customers. Our mission is to help you meet current and future target protein demand and clear the path to commercialization to help you stay at the forefront of protein design and engineering. If you interested in our services, please contact us for more details.

Reference

  1. Fairman, J. W.; et al. The structural biology of β-barrel membrane proteins: a summary of recent reports. Curr Opin Struct Biol. 2011, 21(4): 523-31.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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