β-Sheet Surface Protein Design

Creative Biolabs is recognized as the world leader for providing the most diverse portfolio of protein design solutions for worldwide customers. We have completed a series of challenges in the past few years. In particular, we have established an advanced computer-based β-sheet surface protein design platform which enables us to offer a series of high-quality services for our worldwide customers.

Introduction to β-Sheet Surface Protein

The β-sheet is a common domain in proteins that plays a key role in mediating protein stability. Recent reports of several groups have shown that the β-sheet can interact with a wide variety of residues, such as solvent-facing residues and β-strands residues. In this condition, more and more researchers are dedicated to understanding the structure and biological features that contribute to β-sheet protein stability. For example, the data suggests that aromatic and glutamate pairs have been broadly used for designing and optimizing β-sheet surface proteins. Meanwhile, there has been considerable effort aiming at re-designing β-sheets that are embedded into folded proteins. Computer-based β-sheet protein design has been considered as a powerful tool for improving sequences based on different types of factors, including hydrogen bonding, electrostatics. The β-sheet surface of TNfn3 has been redesigned by the Rosetta model to optimize protein folding and stability. Moreover, recent studies have indicated that mutations on β-sheet sequences are critical to alter the energy functions of various proteins.

The β-Sheet Surface Protein Design. Fig.1 The β-Sheet Surface Protein Design.


Protein design has been regarded as an effective way to analyze protein structure. Most design efforts have been made for the modified proteins containing the β-sheet surface. Here, Creative Biolabs offers many services to test and develop methods for β-sheet surface protein design. In our computer-based platform, the Rosetta model is commonly used to design α/β protein and stabilize β-sheet proteins. Among them, TNfn3 has been incorporated into β-sheet surface for protein folding and stability. In addition, we also provide a range of approaches to insert salt bridges into neighboring strands of β-sheet surface. These methods can specific pair β-strands with others, which is significant for tertiary folds in protein design. Up to now, we have accumulated extensive experience from the accomplishment of different β-sheets surface protein design projects, during which we have received a lot of recognition and praise from our clients. Therefore, our platform should be beneficial to optimizing the function and structure of β-sheet surface and improving the efficacy and functions in different types of disease treatments.

Surface exposed β-sheet residues for the various designs. Fig.2 Surface exposed β-sheet residues for the various designs. (Kim, 2016)

Creative Biolabs is a professional expert in providing various protein design solutions for our clients all over the world. We are specialized in designing and performing high-quality custom assays, with different formats, endpoints, parameters, to satisfy any specific requirement at a most competitive price. For more detailed information, please feel free to contact us or directly sent us an inquiry.


  1. Kim, D.N.; et al. Boosting protein stability with the computational design of β-sheet surfaces. Protein Sci. 2016, 25(3): 702-10.

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