Recombinant Anti-Ad fiber knob x Anti-EPCAM Bispecific Antibody (IgG(L)-scFv) is formed by an IgG and two scFv moieties. The scFv fragments from an anti-Ad fiber knob antibody are fused to the C terminus of an anti-EPCAM IgG light (L) chains, or vice versa. It is a type of appended IgGs, which is bispecific and tetravalent (2+2 antigen-binding valency). This BsAb can retarget of adenoviral vectors to tumour cells. It can be used in cancer gene therapy to transfer a therapeutic gene into tumour cells aiming at selective and efficient cell killing.