Recombinant Anti-Ad fiber knob x Anti-EPCAM Bispecific Antibody (scFv-Fc) is formed by fusing two different scFv fragments to the hinge and Fc regions, in which the Fc region are engineered by KIH. Two of scFv moiety has the antibody variable domain of either anti-Ad fiber knob specificity or anti-EPCAM specificity, which produces bivalent IgG-like molecules with desired characteristics of intact antibodies. This BsAb can retarget of adenoviral vectors to tumour cells. It can be used in cancer gene therapy to transfer a therapeutic gene into tumour cells aiming at selective and efficient cell killing.
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