Recombinant Anti-CA72-4 x Anti-CD3 Bispecific Antibody (Diabody-CH3) is a fusion protein of two diabodies connected together by a CH3 region. In a diabody, the two scFvs with short linkers are from anti-CA72-4 and anti-CD3 antibodies. The VH and VL domains form an inter-chain pairing. The diabody with different specificities is fused to each of the CH3 chains, which results in 2+2 antigen-binding valency and extends the half-life of this BsAb. This BsAb can retarget T cells to tumor cells. It is designed for the research of Gastrointestinal cancer; Ovarian cancer; Colon cancer; Gastric cancer; Breast cancer; Adenocarcinomas therapy.