Recombinant Anti-CA72-4 x Anti-CD3 Bispecific Antibody (Fab-Fv) is designed to be expressed as two polypeptide chains. The heavy chain Fd fragment and light chain of an anti-CA72-4 antibody are fused with the VL and VH domains of an anti-CD3 antibody respectively. It can form natural in vivo heterodimerization. This BsAb can retarget T cells to tumor cells. It is designed for the research of Gastrointestinal cancer; Ovarian cancer; Colon cancer; Gastric cancer; Breast cancer; Adenocarcinomas therapy.