Recombinant Anti-CD28 x Anti-EPCAM Bispecific Antibody (scFv-CH3) contains two chains. The anti-CD28 scFv is fused to the Knob-CH3 domain via a hinge region and the same as the anti-EPCAM scFv to the Hole-CH3 domain, or vice versa. The KIH CH3 domains can facilitate to form a heterodimer and this bivalent binding leads to improvement in avidity. This BsAb can retarget T cells to tumor cells. It is designed for the research of Melanoma; Glioblastoma multiforme (GBM); Non-small cell lung (NSCLC); Colorectal cancer (CRC); Solid tumors therapy.