Recombinant Anti-CD28 x Anti-EPCAM Bispecific Antibody (scFv-IgG) is formed by an IgG and two scFv fragments. The scFvs from an anti-CD28 antibody variable domain are respectively connected to the N terminus of the anti-EPCAM IgG heavy chains or light chains to form scFv-(H)IgG or scFv-(L)IgG. It is a type of appended IgGs and has 2+2 antigen-binding valency. This BsAb can retarget T cells to tumor cells. It is designed for the research of Melanoma; Glioblastoma multiforme (GBM); Non-small cell lung (NSCLC); Colorectal cancer (CRC); Solid tumors therapy.