Recombinant Anti-CD45 x Anti-Myosin Bispecific Antibody (scFv-Fc-scFv) is designed to be expressed as one chain, then assembled to a homodimer of bivalent scFv fragments and the Fc region of IgG. The anti-CD45 scFv is fused to the N-terminus of Fc via a hinge region while another anti-Myosin scFv is linked to the C-terminus of Fc. This scFv-Fc-scFv format has 2+2 antigen-binding valency by employing different scFv fragments. This BsAb can recruit stem cells to infarcted myocardium. It is designed for the research of Myocardial infarction therapy.
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