Recombinant Anti-CD64 x Anti-EGFR Bispecific Antibody (Fab-VHH-Fc) requires expression of three polypeptide chains, encoding two heavy chains (Fc-containing chains) and one light chain, respectively. The Fab of anti-CD64 antibody variable domain is fused to the Fc-knob domain with a hinge region and the same as the VHH of anti-EGFR to the Fc-hole domain, or vice versa. The Fab-Fc and VHH-Fc fragments can be paired via the interaction of the Fc regions. This BsAb can retarget neutrophils cells to tumor cells. It is designed for the research of Head and neck cancer; Colorectal cancer (CRC); Glioblastoma multiforme (GBM); Squamous cancer; Glioblastoma; Medulloblastoma; Non-small cell lung cancer (NSCLC); Renal cell cancer (RCC); Solid tumors therapy.