Recombinant Anti-CEA x Anti-TNFα Bispecific Antibody (scFv-Fc-scFv) is designed to be expressed as one chain, then assembled to a homodimer of bivalent scFv fragments and the Fc region of IgG. The anti-CEA scFv is fused to the N-terminus of Fc via a hinge region while another anti-TNFα scFv is linked to the C-terminus of Fc. This scFv-Fc-scFv format has 2+2 antigen-binding valency by employing different scFv fragments. This BsAb can retarget cytokine (TNFα) to tumor cells. It is designed for the research of Breast cancer; Ovary cancer; Colon cancer; Lung cancer therapy.
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