Recombinant Anti-CEA x Anti-TNFα Bispecific Antibody (VHH-Fc-VHH, Tetravalent HCAb) is a fusion protein of two diabodies connected together by an Fc region. In a diabody, the two VHHs with short linkers are from anti-CEA and anti-TNFα antibodies. The VH and VL domains form an inter-chain pairing. The diabody with different specificities is fused to the Fc fragment, which results in 2+2 antigen-binding valency and exerts Fc-mediated functions or just extends the half-life. This BsAb can retarget cytokine (TNFα) to tumor cells. It is designed for the research of Breast cancer; Ovary cancer; Colon cancer; Lung cancer therapy.