Recombinant Anti-DNMT1 x Anti-SHH Bispecific Antibody (scDiabody-Fc) is a fusion protein of two scDiabodies with an Fc region. In single-chain Diabodies, an additional connecting linker is introduced between chains of the anti-DNMT1/anti-SHH diabody. The scDiabody with two specificities is further fused to the Fc domain to form BsAb with 2+2 antigen-binding valency. This BsAb can bind two factors simultaneously, block Hedgehog binding to PTCH, downregulate of DNMT1 and block DNA methylation. It is designed for the research of Hormone refractory breast cancer (HRBC) therapy.
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