Recombinant Anti-DNMT1 x Anti-SHH Bispecific Antibody (scFv-CH1/CL) is designed to be expressed as two chains. One scFv from an anti-DNMT1 antibody variable domain is fused to CH1 and the other scFv from an anti-SHH antibody variable domain fused to CL, or vice versa. And the CH1 and CL domains are linked covalently via a natural disulfide bond. This BsAb can bind two factors simultaneously, block Hedgehog binding to PTCH, downregulate of DNMT1 and block DNA methylation. It is designed for the research of Hormone refractory breast cancer (HRBC) therapy.
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