Recombinant Anti-EGFR x Anti-HER3 Bispecific Antibody (DART) is designed to be expressed as two polypeptide chains respectively, and a disulfide bond is introduced into the chains. The two chains are cross-over pairing to form a dimer. It contains a His-tag for affinity purification. This BsAb can bind and inactivate two receptors simultaneously. It is designed for the research of Solid tumors therapy.