Recombinant Anti-FGF2 x Anti-VEGF Bispecific Antibody (scFv-Fc-scFv) is designed to be expressed as one chain, then assembled to a homodimer of bivalent scFv fragments and the Fc region of IgG. The anti-FGF2 scFv is fused to the N-terminus of Fc via a hinge region while another anti-VEGF scFv is linked to the C-terminus of Fc. This scFv-Fc-scFv format has 2+2 antigen-binding valency by employing different scFv fragments. This BsAb can bind and inactivate two ligands simultaneously. It is designed for the research of Hepatocellular cancer (HCC); Breast cancer; Colon cancer; Lung cancer; Glioma cancer therapy.
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