Recombinant Anti-FGFR1 x Anti-KLB Bispecific Antibody (scFv-Fc-scFv) is designed to be expressed as one chain, then assembled to a homodimer of bivalent scFv fragments and the Fc region of IgG. The anti-FGFR1 scFv is fused to the N-terminus of Fc via a hinge region while another anti-KLB scFv is linked to the C-terminus of Fc. This scFv-Fc-scFv format has 2+2 antigen-binding valency by employing different scFv fragments. This BsAb can bind two factors simultaneously and act as mimetic hormone (FGF21) . It is designed for the research of Type 2 diabetes therapy.