Recombinant Anti-FGFR1 x Anti-KLB Bispecific Antibody (scFv-IgG) is formed by an IgG and two scFv fragments. The scFvs from an anti-FGFR1 antibody variable domain are respectively connected to the N terminus of the anti-KLB IgG heavy chains or light chains to form scFv-(H)IgG or scFv-(L)IgG. It is a type of appended IgGs and has 2+2 antigen-binding valency. This BsAb can bind two factors simultaneously and act as mimetic hormone (FGF21) . It is designed for the research of Type 2 diabetes therapy.