Recombinant Anti-gp40 x Anti-TFRC Bispecific Antibody (Diabody) is designed to be expressed as two polypeptide chains respectively. The two chains are cross-over pairing to form a dimer. It contains a His-tag for affinity purification. This BsAb can bind two tumor antigens simultaneously. It is designed for the research of Colon cancer; Ovarian cancer therapy.