Recombinant Anti-gp40 x Anti-TFRC Bispecific Antibody (IgG(L)-scFv) is formed by an IgG and two scFv moieties. The scFv fragments from an anti-gp40 antibody are fused to the C terminus of an anti-TFRC IgG light (L) chains, or vice versa. It is a type of appended IgGs, which is bispecific and tetravalent (2+2 antigen-binding valency). This BsAb can bind two tumor antigens simultaneously. It is designed for the research of Colon cancer; Ovarian cancer therapy.