Recombinant Anti-gp40 x Anti-TFRC Bispecific Antibody (Miniantibody) is a fusion protein of two scFv fragments combined with distinct specificity. One scFv of an anti-gp40 antibody variable domain is linked to another scFv of an anti-TFRC antibody variable domain by a flexible hinge region. This BsAb format has the decreased size and better penetration than conventional IgGs in the clinical diagnostics and potentially therapy. This BsAb can bind two tumor antigens simultaneously. It is designed for the research of Colon cancer; Ovarian cancer therapy.