Recombinant Anti-gp40 x Anti-TFRC Bispecific Antibody (scFv-CH3) contains two chains. The anti-gp40 scFv is fused to the Knob-CH3 domain via a hinge region and the same as the anti-TFRC scFv to the Hole-CH3 domain, or vice versa. The KIH CH3 domains can facilitate to form a heterodimer and this bivalent binding leads to improvement in avidity. This BsAb can bind two tumor antigens simultaneously. It is designed for the research of Colon cancer; Ovarian cancer therapy.