Recombinant Anti-gp40 x Anti-TFRC Bispecific Antibody (Tandem scFv-Fc) is designed to be expressed as a homodimer, which has 2+2 antigen-binding valency. This format consists of one Fc region and two tandem scFv fragments, in which the anti-gp40/TFRC tandem scFv is fused to the Fc domain. The engineered fragment format is used to extend the half-life. This BsAb can bind two tumor antigens simultaneously. It is designed for the research of Colon cancer; Ovarian cancer therapy.