Recombinant Anti-HER2 x Anti-VEGF Bispecific Antibody (Fab-Fv) is designed to be expressed as two polypeptide chains. The heavy chain Fd fragment and light chain of an anti-HER2 antibody are fused with the VL and VH domains of an anti-VEGF antibody respectively. It can form natural in vivo heterodimerization. This BsAb can bind two factors simultaneously and inhibit cell proliferation. It is designed for the research of Colorectal cancer; Breast cancer; Lung cancer; Renal cell cancer (RCC); Glioma cancer; Glioblastoma; Ovarian cancer therapy.