Recombinant Anti-HER2 x Anti-VEGF Bispecific Antibody (Fab-VHH-Fc) requires expression of three polypeptide chains, encoding two heavy chains (Fc-containing chains) and one light chain, respectively. The Fab of anti-HER2 antibody variable domain is fused to the Fc-knob domain with a hinge region and the same as the VHH of anti-VEGF to the Fc-hole domain, or vice versa. The Fab-Fc and VHH-Fc fragments can be paired via the interaction of the Fc regions. This BsAb can bind two factors simultaneously and inhibit cell proliferation. It is designed for the research of Colorectal cancer; Breast cancer; Lung cancer; Renal cell cancer (RCC); Glioma cancer; Glioblastoma; Ovarian cancer therapy.