Recombinant Anti-HER2 x Anti-VEGF Bispecific Antibody (Miniantibody) is a fusion protein of two scFv fragments combined with distinct specificity. One scFv of an anti-HER2 antibody variable domain is linked to another scFv of an anti-VEGF antibody variable domain by a flexible hinge region. This BsAb format has the decreased size and better penetration than conventional IgGs in the clinical diagnostics and potentially therapy. This BsAb can bind two factors simultaneously and inhibit cell proliferation. It is designed for the research of Colorectal cancer; Breast cancer; Lung cancer; Renal cell cancer (RCC); Glioma cancer; Glioblastoma; Ovarian cancer therapy.