Recombinant Anti-Kit x Anti-VCAM-1 Bispecific Antibody (F(ab')2-scFv2) is designed to be expressed as two chains combined via a hinge region. The scFv of the anti-Kit specificity is fused to the C terminus of the anti-VCAM-1 Fab fragment, or vice versa. This format comprises four binding sites (two same scFv regions and two same Fab fragments), two for each antigen. This BsAb can retarget hematopoietic stem cells toinjured myocardium. It is designed for the research of Cardiovascular disease therapy.