Recombinant Anti-Kit x Anti-VCAM-1 Bispecific Antibody (IgG-Fv) is formed by an Immunoglobulin G (IgG) and a fragment variable Fv region, in which the Fc of IgG is engineered by KIH. The Fv region of an anti-Kit antibody variable domain is fused to the C terminal of an IgG of anti-VCAM-1 antibody, or vice versa. The format retains desired characteristics of intact antibodies. This BsAb can retarget hematopoietic stem cells toinjured myocardium. It is designed for the research of Cardiovascular disease therapy.