Recombinant Anti-pre S2 protein x Anti-S protein Bispecific Antibody (scFv-IgG) is formed by an IgG and two scFv fragments. The scFvs from an anti-pre S2 protein antibody variable domain are respectively connected to the N terminus of the anti-S protein IgG heavy chains or light chains to form scFv-(H)IgG or scFv-(L)IgG. It is a type of appended IgGs and has 2+2 antigen-binding valency. This BsAb can bind two antigens simultaneously. It is designed for the research of HBV infection therapy.