Recombinant Anti-TG x Anti-TPO Bispecific Antibody (Miniantibody) is a fusion protein of two scFv fragments combined with distinct specificity. One scFv of an anti-TG antibody variable domain is linked to another scFv of an anti-TPO antibody variable domain by a flexible hinge region. This BsAb format has the decreased size and better penetration than conventional IgGs in the clinical diagnostics and potentially therapy. This BsAb can bind two antigens simultaneously and block the synthesis of thyroid hormones. It is designed for the research of Autoimmune thyroid disease therapy.