Albumin belongs to globular proteins. Human serum albumin (HSA) is the most abundant in plasma. It is a monomeric macromolecule with the weight of about 66 kDa. The gene governing HSA synthesis is located on the long arm of chromosome 4, near the centromere at position q11–22. HSA regulates the oncotic pressure (colloid osmotic pressure) of blood plasma. HAS acts as the main carrier for exogenous drugs and fatty acids, and can also be used as a biomarker of some diseases, such as cancer, ischemia, rheumatoid arthritis, etc. TNF (Tumor necrosis factor), also known as Cachectin, TNF-alpha, TNFA, TNFSF2 (Tumor necrosis factor ligand superfamily member 2), TNLG1F or DIF, is a 25.6 kDa proinflammatory cytokine that can stimulate the acute phase reaction and cause cell death or apoptosis. It has 233 amino acids encoded by the gene located at the chromosome 6p21.33. This factor is a member of the tumor necrosis factor superfamily (TNFSF), which can regulate many biological processes, including cell proliferation and differentiation, apoptosis, coagulation and lipid metabolism. It is a type of cytotoxin chiefly produced by activated macrophages, though can be expressed by other cells as well. This cytokine is involved in systemic inflammation and implicated in tumor regression, cachexia, and septic shock. And TNF functions through binding its receptors TNFRSF1A and TNFRSF1B.