Alkaline Phosphatase (AP) or basic phosphatase is a homodimeric, membrane-derived glycoprotein of 86 kilodaltons from a bovine intestine. Each monomer of this protein enzyme consist of five cysteine residues, two zinc atoms, and one magnesium atom. The structure contributes to catalytic function of enzyme and optimally active at alkaline pH environments. The abnormal levels of AP in the blood might be associated with the liver, gall bladder or bones issues. Additionally, Kidney tumors, infections and malnutrition have been detected abnormal level of alkaline phosphatase in blood. AP is specific for a broad of molecules including phosphate esters of alcohols, amines, phenols, and pyrophosphates. AP is commonly applied to dephosphorylate proteins and nucleic acids. CEA (Carcinoembryonic antigen) is a type of glycoprotein attached to the cell membrane via glycosylphosphatidylinositol (GPI) anchor and stands for a set of highly related substance implicated in cell adhesion. It belongs to the immunoglobulin superfamily (IgSF) and there are two immunoglobulin domains including an N-terminal domain of 108 amino acids homologous to IgV-like and six domains homologous to the IgC2-like. The CAE gene family in humans is clustered on chromosome 19q and comprises 29 genes, 18 of which are commonly found. CEA is generally expressed in gastrointestinal tissue during embryonic development, but produced in very small amounts after birth. The serum level of it is not raised unless certain cancers tested. The CEA has become one of the most widely used tumor markers in clinical diagnosis and its main application is in gastrointestinal cancers, particularly in colorectal malignancy.