CEA (Carcinoembryonic antigen) is a type of glycoprotein attached to the cell membrane via glycosylphosphatidylinositol (GPI) anchor and stands for a set of highly related substance implicated in cell adhesion. It belongs to the immunoglobulin superfamily (IgSF) and there are two immunoglobulin domains including an N-terminal domain of 108 amino acids homologous to IgV-like and six domains homologous to the IgC2-like. The CAE gene family in humans is clustered on chromosome 19q and comprises 29 genes, 18 of which are commonly found. CEA is generally expressed in gastrointestinal tissue during embryonic development, but produced in very small amounts after birth. The serum level of it is not raised unless certain cancers tested. The CEA has become one of the most widely used tumor markers in clinical diagnosis and its main application is in gastrointestinal cancers, particularly in colorectal malignancy. Tumour necrosis factor alpha (TNFα) belongs to the TNF superfamily, a 233-amino acid-long type II transmembrane protein. It is primarily secreted by activated macrophages and activated T lymphocytes. TNFα is referred as the first inflammatory cytokine-inducing complex immune responses and capable of replicating the ability of endotoxin in inducing haemorrhagic tumour necrosis. It has pleiotropic properties such as tumour cell apoptosis/necrosis and extensive thrombosis and destruction of tumour vasculature. TNFα plays a main role in cancer treatment and exerts anti-tumour activity and also perform anti-cancer effects.