Recombinant Anti-CD20 x Anti-CD22 Tandem Fab-NGB immunotoxin (Bispecific Immunotoxin) is designed to be expressed as the Nigrin b (NGB) fused to a tandem Fab recognizing CD20 and CD22. It contains three chains and a His-tag at the C/N terminus for affinity purification. The two Fd regions are connected by a flexible linker. This bispecific immunotoxin can deliver NGB to antigen-specific cells. Then NGB is a lectin and can kill the target cells. The bispecific immunotoxin is designed for the research of Lymphoma; Leukemia; Antoimmune disease; Immune dysfunction disease therapy.