Recombinant Anti-CTLA4 x Anti-GLUT2 Tandem Fab-PAP immunotoxin (Bispecific Immunotoxin) is designed to be expressed as the pokeweed antiviral protein (PAP) fused to a tandem Fab recognizing CTLA4 and GLUT2. It contains three chains and a His-tag at the C/N terminus for affinity purification. The two Fd regions are connected by a flexible linker. This bispecific immunotoxin can deliver PAP to antigen-specific cells. Then PAP is a ribosome inhibitory single chain plant toxin and can kill the target cells. The bispecific immunotoxin is designed for the research of Renal cell cancer (RCC) therapy.
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