Butyrylation Specific Antibody Discovery Service

Background Antibody Types Discovery Strategy Types of PTM Why Choose Us? FAQs

Creative Biolabs offers Butyrylation-Specific Antibody Discovery Services to deliver precise and sensitive antibodies for research advancement using our specialized antibody production and characterization skills. Our solutions enable you to manage the challenges that come with studying this crucial post-translational modification.

Background

Butyrylation serves as a novel post-translational modification where a butyryl group attaches to lysine residues in proteins. The structural resemblance between butyrylation and other chemical modifications, such as acetylation and propionylation, together with its existence in both prokaryotic and eukaryotic life forms and its influence on multiple protein functions, has become the primary target of recent scientific investigations.

Fig. 1 Lysine butyrylome in 5-FU-resistant cell line KYSE150-FR and the parental cells KYSE150. (OA Literature) Fig.1 Scheme of the lysine butyrylome in 5-FU-resistant cell line KYSE150-FR and the parental cells KYSE150.¹

Butyrylation, alongside comparable PTMs, broadens protein functionality while affecting various cellular processes. Histone lysine butyrylation acts as an epigenetic regulator that modifies chromatin structure to control DNA transcription and cellular metabolism. Inappropriate butyrylation of proteins is linked to the onset of metabolic diseases and cancer formation. Research into butyrylation processes is necessary to grasp how cellular functions operate and how diseases develop. The analysis of PTM requires specific antibodies to allow researchers to detect modified proteins and locate modification sites, as well as understand the butyrylation effects. The services offered by Creative Biolabs provide indispensable tools that researchers need to move forward with their research.

Antibody Types

Creative Biolabs delivers both monoclonal and polyclonal antibodies derived from rabbit, mouse, or sheep host animals. Our antibody types include:

Site-Specific Anti-Butyrylation Antibodies: The antibodies are engineered to detect butyrylation at specific lysine residues in proteins. These antibodies enable precise investigation of specific butyrylation sites and allow distinction between various butyrylation events.
Pan Anti-Butyryllysine Antibodies: The antibodies will detect any protein that contains butyrylated lysine residues, no matter what modification site is present. These antibodies enable scientists to measure total protein butyrylation levels and discover new proteins that undergo butyrylation.

Discovery Strategy

Creative Biolabs uses three main strategies to develop our antibody services:

Monospecific Anti-Butyrylation Polyclonal Antibody Production: The classic approach to antibody production requires immunization of animals such as rabbits, mice, or sheep using butyrylated antigens which are usually butyrylated proteins or peptides. High specificity for butyrylated lysine residues emerges from purification of the resulting polyclonal antibodies. This method effectively produces antibodies that identify specific butyrylation modifications.

Fig. 2 General process of monospecific anti-butyrylation polyclonal antibody production. (Creative Biolabs Original)

Phage Display Strategy for Anti-Butyrylation Monoclonal Antibody Discovery: Generating monoclonal antibodies can be effectively accomplished through the use of phage display. The antibody libraries bind to bacteriophage surfaces, and those phages that show high-affinity antibodies towards the butyrylated target get selected. The technique enables quick discovery of monoclonal antibodies that exhibit high specificity.

Fig. 3 General process of phage display for anti-butyrylation monoclonal antibody discovery. (Creative Biolabs Original)

Hybridoma Strategy for Anti-Butyrylation Monoclonal Antibody Discovery: Hybridoma technology generates hybrid cells by fusing B cells from immunized animals with myeloma cells. The hybridomas generate monoclonal antibodies that recognize only the butyrylated antigen. The production of monoclonal antibodies through this method ensures both high specificity and strong affinity.

Fig. 4 General process of hybridoma for anti-butyrylation monoclonal antibody discovery. (Creative Biolabs Original)

Types of PTM

We provide butyrylation specific antibodies and antibodies targeting a variety of PTMs, as shown in the table below.

Why Choose Us?

Choose Creative Biolabs as your trusted provider for Butyrylation-Specific Antibody Discovery Services. We offer:

High Specificity and Sensitivity: Through our proprietary High-Affi™ technology, our antibodies maintain minimal cross-reactivity with unmodified peptides as well as acetylated or other acylated variants. You achieve dependable and precise experimental outcomes through this process.
Comprehensive Service Portfolio: Our laboratory supplies both pan and site-specific anti-butyrylation antibodies to fulfill diverse research requirements. Our solutions cover both general butyrylation levels research and targeted modification site analysis.
Customization: We recognize that research projects differ from one another. Creative Biolabs provides customized services to fulfill your unique requirements by offering antigen design along with antibody production and purification.
Expertise and Experience: Our Ph.D.-level scientists bring extensive experience in antibody discovery and production to provide you with high-quality antibodies for your research needs.
Proven Track Record: Published data demonstrates that our services have enabled many successful research projects.

FAQs

Q: What criteria should I use to determine whether my research needs a butyrylation-specific antibody?

A: When your study requires the analysis of butyrylation's impact within biological processes or involves identifying proteins with butyrylation modifications and mapping their sites, then specialized butyrylation antibodies are necessary. Our team specializes in guiding researchers to select the most appropriate antibody for their particular application needs. Please reach out to us to schedule a consultation.

Q: What is the main difference between your monoclonal antibodies and polyclonal antibodies?

A: Monoclonal antibodies exhibit high specificity toward a single epitope, which provides reliability and replicability in research experiments. Polyclonal antibodies maintain specificity yet recognize multiple epitopes which may result in experimental variability. Our comprehensive range includes both monoclonal and polyclonal antibodies to accommodate diverse research requirements. Contact us to discuss your specific requirements.

Q: What antibodies do you produce against various post-translational modifications (PTMs)?

A: Creative Biolabs features a complete range of PTM-specific antibody production services that cover acetylation, phosphorylation, and methylation, along with butyrylation-specific antibodies. We welcome any questions regarding our complete service offerings.

Q: How should I choose a butyrylation-specific antibody?

A: When selecting an antibody, you should evaluate its specificity alongside its sensitivity and intended use cases, such as Western blotting, immunoprecipitation, and immunofluorescence. Our detailed product information and expert support will guide you toward the best decision. Contact our team for personalized guidance.

Q: How can I contact you for antibody production services?

A: Getting started is easy! Connect with us by visiting our website or using the contact details listed below this text. Our team stands ready to discuss your project needs and provide a comprehensive quote while guiding you throughout each step. Our primary objective is to assist you in achieving research success.

Creative Biolabs' services for discovering butyrylation-specific antibodies equip researchers with essential tools to investigate the significant field of protein butyrylation. Our expertise in antibody production and characterization enables us to support your research objectives. Please contact us for more details.

Reference

He, Yan, et al. "Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance." Cell Discovery 9.1 (2023): 74. Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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