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CACNA1S Membrane Protein Introduction

Introduction of CACNA1S

CACNA1S, calcium channel, voltage-dependent, L type, alpha 1S subunit, also known as CACNA1S or Cav1.1, is a protein which in humans is encoded by the CACNA1S gene. It is also known as CACNL1A3 and dihydropyridine receptor - DHPR, this naming is due to its blocking action to DHP. It is one of the five subunits of L type voltage-dependent calcium channel. Mutations in this gene are associated with hypokalemic periodic paralysis, thyrotoxic periodic paralysis, and malignant hyperthermia susceptibility.

Basic Information of CACNA1S
Protein Name Voltage-dependent L-type calcium channel subunit alpha-1S
Gene Name CACNA1S
Aliases Calcium channel, L type, alpha-1 polypeptide, isoform 3, Voltage-gated calcium channel subunit alpha Cav1.1, CACH1, CACN1, CACNL1A3
Organism Homo sapiens (Human)
UniProt ID Q13698
Transmembrane Times 28
Length (aa) 1873
Sequence MEPSSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLDEDLRGYMSWITQGEVMDVEDFREGKLSLDEGGSDTESLYEIAGLNKIIQFIRHWRQWNRIFRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGIMAYGGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYIVTSSYFEYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLMAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISSAFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDIVQIQAGLRTIEEEAAPEICRTVSGDLAAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEEMESPVFLEDFPQDPRTNPLARANTNNANANVAYGNSNHSNSHVFSSVHYEREFPEETETPATRGRALGQPCRVLGPHSKPCVEMLKGLLTQRAMPRGQAPPAPCQCPRVESSMPEDRKSSTPGSLHEETPHSRSTRENTSRCSAPATALLIQKALVRGGLGTLAADANFIMATGQALADACQMEPEEVEIMATELLKGREAPEGMASSLGCLNLGSSLGSLDQHQGSQETLIPPRL

Function ofCACNA1S Membrane Protein

CACNA1S is also called Cav1.1. This kind of voltage-sensitive L type calcium channel mediates the calcium influx in skeletal muscle. It encodes alpha-1S subunit (one of the five subunits) of the slowly inactivating L type voltage-dependent calcium channel. CACNA1S plays an important role in excitation-contraction coupling in skeletal muscle via their interaction with RYR1, which can trigger calcium release from the sarcoplasmic reticulum, then finally results in muscle contraction. The mutations of this gene are associated with the following diseases: hypokalemic periodic paralysis, thyrotoxic periodic paralysis, and malignant hyperthermia susceptibility.

Working model of CACNA1S, Fig.1 Working model of CACNA1S, (Jorquera, 2013)

Application of CACNA1S Membrane Protein in Literature

  1. Schartner V., et al. Dihydropyridine receptor (DHPR, CACNA1S) congenital myopathy. Acta Neuropathol. 2017, 133(4): 517-533. PubMed ID: 28012042

    This article indicates that DHPR is important for perinatal muscle function in human, and the studies demonstrate that CACNA1S and ECC can be considered as therapeutic targets for the development of treatments.

  2. Schiemann A.H and Stowell K.M. Comparison of pathogenicity prediction tools on missense variants in RYR1 and CACNA1S associated with malignant hyperthermia. Br J Anaesth. 2016, 117(1): 124-8. PubMed ID: 27147545

    This article predicts the impact of a missense variant on RYR1 and CACNA1S function, and suggests that the prediction should be a combination with other available data including functional assays and segregation analysis.

  3. Zhang T., et al. Calpain inhibition rescues troponin T3 fragmentation, increases Cav1.1, and enhances skeletal muscle force in aging sedentary mice. Aging Cell. 2016, 15(3): 488-98. PubMed ID: 26892246

    This study demonstrates that fast skeletal muscle troponin T3 can regulate transcription of Cacna1s, also known as Cav1.1. Calpain inhibitor can prevent TnT3 fragmentation, and cause CACNA1S downregulation and finally improve the muscle fore in aging sedentary mice.

  4. Fiszer D., et al. Next-generation Sequencing of RYR1 and CACNA1S in Malignant Hyperthermia and Exertional Heat Illness. Anesthesiology. 2015, 122(5): 1033-46. PubMed ID: 25658027

    This article provides the next-generation sequencing which is efficient at identifying diagnostically useful and potentially important variants in RYR1and CACNA1S in Malignant Hyperthermia and Exertional Heat Illness.

  5. Polster A., et al. Stac adaptor proteins regulate trafficking and function of muscle and neuronal L-type Ca2+ channels. Proc Natl Acad Sci U S A. 2015, 112(2): 602-6. PubMed ID: 25548159

    This article reveals that Stac3 is a necessary chaperone of CACNA1S in skeletal muscle via regulating trafficking and function of calcium channel.

CACNA1S Preparation Options

We provide custom membrane protein preparation services for worldwide customers. Leveraging by our advanced Magic™ membrane protein production platform, we are able to present target membrane protein in multiple active formats. Our professional scientists are happy to help you find an ideal method and make your project a success. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-CACNA1S antibody development services.


Creative Biolabs provides high-quality membrane protein preparation service to facilitate the development of worldwide customer’s research. During the past years, we have successfully established a powerful Magic™ membrane protein platform which enables us to provide a series of membrane protein preparation services. For more detailed information, please feel free to contact us.

Reference

  1. Jorquera G, et al. (2013). Cav1.1 controls frequency-dependent events regulating adult skeletal muscle plasticity. J Cell Sci. 126(Pt 5):1189-98.

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