The cadherin epidermal growth factor (EGF) laminin G (LAG) seven-pass G-type receptors (CELSRs) are a special sub-group of adhesion G protein-coupled receptors. CELSR proteins have a large ectodomain (~2700 amino acids), followed by seven transmembrane segments and a cytoplasmic tail that varies in size. CELSR is broadly expressed in the nervous system from embryonic and early post-natal development until the adult stage. Moreover, its expression is regulated spatially and temporally, in complementary patterns. In addition, CELSR expression is also observed in other non-neural systems, such as the skin, lungs, heart, kidney, and spleen, as well as the digestive and reproductive systems.
In the nervous system, CELSRs have been characterized as critical regulators of neuronal migration, dendrite growth and axon guidance, vertebrate planar cell polarity, and neuronal tube closure. Moreover, they have played a role in pancreas β cell differentiation and glucose homeostasis. Mutations in the ectodomain or other gene locations of CELSRs are associated with neural tube defects and other diseases in humans, such as tumors and cardiovascular diseases.
However, many of the signaling pathways and regulatory mechanisms remain elusive. Future studies using more advanced biology or biochemical tools may provide further insights into the mechanisms underlying CELSR function, laying the foundation for the design of new CELSR-targeted therapeutic reagents. Here, we give an introduction regarding the recent research advances, structural and functional studies of human cadherin EGF LAG CELSR family members.
To aid in the in vitro studies of these membrane proteins, Creative Biolabs utilizes various approaches to extract and isolate your membrane protein of interest from the biological membranes and reconstitute the targets into different systems. We have a powerful Magic™ membrane protein production platform, which enables protein reconstitution into not only conventional systems such as detergent micelles, and proteoliposomes but also novel, more native-like systems such as nanodiscs, lipoparticles, and polymers. The latter is often required to better preserve their structural integrity and functional activity, thus making it possible for more advanced investigations.
Meanwhile, our Magic™ membrane protein antibody discovery platform offers antibody development services against these membrane proteins. We can deliver these services in packages or separately. We also present DNA immunization service for anti-membrane protein antibody development. Please feel free to contact us for more information.