Anti-CD276 (CBFYC-1202) h(41BB-CD3ζ) CAR, pCDCAR1 vector

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Details

Target
CD276

Targeting Cell Type
T cell

Targeting Diseases
Skin cancer (SCC)

Generation
Second

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-41BB-CD3ζ

Discription of Signaling Cassetes
41BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells and binds to a high-affinity ligand (4-1BB) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ, ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMS, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound.CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

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Published Data

Complete CAR data FuncS

Fig.1 Therapeutic effects of B7-H3 CAR-T cells in tumor xenografts.

CAR Construction : 8H9-4-1BB-CD3ζ

Fig.1 Therapeutic effects of B7-H3 CAR-T cells in tumor xenografts.

Tumor growth in A549-injected mice (b) or HCT 116-injected mice (c) was measured using digital caliper, and tumor area was calculated.

Liu, J., Yang, S., Cao, B., Zhou, G., Zhang, F., Wang, Y., ... & Zhao, Q. (2021). Targeting B7-H3 via chimeric antigen receptor T cells and bispecific killer cell engagers augments antitumor response of cytotoxic lymphocytes. Journal of hematology & oncology, 14(1), 1-18.

Complete CAR data FCM

Fig.2 Cytokine production in B7-H3 CAR and vehicle T cells.

CAR Construction : 8H9-4-1BB-CD3ζ

Fig.2 Cytokine production in B7-H3 CAR and vehicle T cells.

Effector T cells were incubated with target A549 or HCT 116 cells at a ratio of 10:1 for 24 h. The concentrations of cytokines were measured using flow cytometry.

Complete CAR data ELISA

Fig.3 Memory cell phenotypes of B7-H3 CAR-T cells.

CAR Construction : 8H9-4-1BB-CD3ζ

Fig.3 Memory cell phenotypes of B7-H3 CAR-T cells.

Expression of CD62L and CCR7 on T cells was identified with staining with anti-CD62L and anti-CCR7 antibodies, respectively, and measured using flow cytometry.

Complete CAR data FCM

Fig.4 Degranulation markers in B7-H3 CAR T cells.

CAR Construction : 8H9-4-1BB-CD3ζ

Fig.4 Degranulation markers in B7-H3 CAR T cells.

Expressions of CD107a, intracellular granzyme B and perforin staining on T cells were stained with anti CD107a, granzyme B and perforin antibodies and measured by flow cytometry.

CAR scFv data SPR

Fig.5 The binding ability of anti-B7-H3 mAb.

CAR Construction :

Fig.5 The binding ability of anti-B7-H3 mAb.

Binding kinetics of anti-B7-H3 antibody ch8H9 to (2Ig-B7-H3-Fc WT, R127A, and D128A) by surface plasmon resonance.

Ahmed, M., Cheng, M., Zhao, Q., Goldgur, Y., Cheal, S. M., Guo, H. F., ... & Cheung, N. K. V. (2015). Humanized affinity-matured monoclonal antibody 8H9 has potent antitumor activity and binds to FG loop of tumor antigen B7-H3. Journal of Biological Chemistry, 290(50), 30018-30029.

More Published Data More Published Data

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-CD276 (CBFYC-1202) h(41BB-CD3ζ) CAR, pCDCAR1 (CAR-WFY2727)

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