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Anti-CD56 (N901) h(CD28-CD3ζ) CAR, pCDCAR1 (CAR-ZP124)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-CD56 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human CD56. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-CD56 antibody linked to CD28 and CD3ζ signaling domains. And the vector product was designed for the treatment of SCLC.

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Details

  • Target
  • CD56
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • SCLC
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-CD28-CD3ζ
  • Discription of Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR T cell containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ, ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • N901
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • Neural cell adhesion molecule
  • Synonyms
  • NCAM1; CD56; MSK39; NCAM; neural cell adhesion molecule 1

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  • Published Data
Complete CAR data Cyt

Fig.1 CD56R-specific CAR T cells kill CD56-expressing targets in vitro.

CAR Construction : N901-CD28-CD3ζ Latest CAR Construction

Fig.1 CD56R-specific CAR T cells kill CD56-expressing targets in vitro.

Chromium release assays of CD56R-CAR+ T cells co-cultured 20:1 with the following targets: Neuroblastoma (CHLA-255, IMR-32, SKN-BE, and SK-N-SH) and SCLC (HTB-119) cell lines; NK-cell leukemia (KHYG1) and glioma/astrocytoma (U87) cell lines.

Crossland, D. L., Denning, W. L., Ang, S., Olivares, S., Mi, T., Switzer, K., ... & Heymach, J. V. (2018). Antitumor activity of CD56-chimeric antigen receptor T cells in neuroblastoma and SCLC models. Oncogene, 37(27), 3686-3697.

Complete CAR data FCM

Fig.2 Anti- or pro-tumor cytokine expression.

CAR Construction : N901-CD28-CD3ζ Latest CAR Construction

Fig.2 Anti- or pro-tumor cytokine expression.

Expression anti- or pro-tumor cytokine of CD4+ and CD8+ CD56R-CAR+ T cells was detected by intracellular flow cytometry after culturing CD3+ CD56R-CAR+ T cells in the diffrent conditions.

Crossland, D. L., Denning, W. L., Ang, S., Olivares, S., Mi, T., Switzer, K., ... & Heymach, J. V. (2018). Antitumor activity of CD56-chimeric antigen receptor T cells in neuroblastoma and SCLC models. Oncogene, 37(27), 3686-3697.

Complete CAR data BI

Fig.3 The ability of CD56R-CAR+T to control tumor growth in vivo.

CAR Construction : Latest CAR Construction

Fig.3 The ability of CD56R-CAR+T to control tumor growth in vivo.

CD56R-CAR+ T cells inhibit the growth of human CD56+ tumors in the high/low tumor-burden CD56+ neuroblastoma mouse model..

Crossland, D. L., Denning, W. L., Ang, S., Olivares, S., Mi, T., Switzer, K., ... & Heymach, J. V. (2018). Antitumor activity of CD56-chimeric antigen receptor T cells in neuroblastoma and SCLC models. Oncogene, 37(27), 3686-3697.

More Published Data More Published Data

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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