pCDCAR1 TYR h(BBζ)(CAR-YJ435)

The vector of anti-TYR chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human TYR. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-TYR antibody linked to 4-1BB (CD137) and CD3ζ signaling domains. And the vector product was designed for the treatment of Melanoma.


Details


Target
TYR
Target Species
Human
Targeting Cell Type
T cell
Targeting Diseases
Melanoma
Generation
Second
Vector Name
pCDCAR1
Vector Length
~8kb
Vector Type
Lentiviral vector
Receptor Construction
scFv-41BB-CD3ζ
Discription of Signaling Cassetes
41BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
Antibody Clone
T311
Antibody Host
Mouse

Target


Gene Name
Tyrosinase
Official Symbol
TYR
Gene ID
UniProt ID
Synonyms
TYR; Tyrosinase; Oculocutaneous Albinism IA; Tumor Rejection Antigen AB; Monophenol Monooxygenase; EC 1.14.18.1; LB24-AB; SK29-AB
Introduction
TYR catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. TYR has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in TYR result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of TYR.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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CONTACT US

USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994
Fax: 1-631-207-8356
Email:

Europe
Tel: 44-207-097-1828

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