pCDCAR1 Mesothelin h(ICOSζ)(CAR-LC037)
The vector of anti-mesothelin chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human mesothelin. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-mesothelin antibody linked to CD3ζ and ICOS (CD278) signaling domains. And the vector product was designed for the treatment of pancreatic cancer, ovarian cancer, lung cancer, malignant pleural mesothelioma tumors (MPM tumors).
Targeting Cell Type
Pancreatic cancer, Ovarian cancer, Lung cancer, Malignant pleural mesothelioma tumors (MPM tumors)
Discription of Signaling Cassetes
CD278 or ICOS (Inducible T-cell COStimulator) is a CD28-superfamily costimulatory molecule that is expressed on activated T cells. It is thought to be important for Th2 cells in particular. ICOS is constitutively expressed on TH17 cells and anti-CD3/ICOS stimulation induced RORγt and T-bet expression in these cells, leading to increased secretion of IL-17A, IL-21, and IFN-γ compared with CD3/CD28 stimulation.
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
MSLN; mesothelin; MPF; SMRP; pancreatic cancer; ovarian cancer; lung cancer; malignant pleural mesothelioma tumors; MPM tumors; scFv; FcεRIγ; Chimeric antigen receptor; T cell; Retrovirus; CARs; Lentivirus; chimeric T cell receptors; chimeric immunoreceptors; chimeric antigen receptors; SS1
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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