Close

pCDCAR2 MUC1×ERBB2 h(BB/ζ) (CAR-LC361)

The vector of anti-MUC1 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human MUC1. The T cells are genetically modified through transduction with a nonviral vector expressing scFv of anti-MUC1 antibody linked to CD3-zeta signaling domain,and another nonviral vector expressing Heirn linked to CD28 and 4-1BB (CD137) signaling domains. When the target cell are double postive with MUC1 and EGFR, he dsCAR modified T cell can proliferate effectively. And the vector product was designed for the treatment of MUC1-expressing tumors and viral infection.

Datasheet   Add to Cart

Details

  • Target
  • MUC1
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • MUC1-expressing tumors, viral infection
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Nonviral
  • Receptor Construction
  • scFv (MUC1)-CD3ζ/Herin-CD28-41BB
  • Discription of Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    41BB
    CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • Pemtumomab
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • Mucin 1 & Erb-b2 receptor tyrosine kinase 2
  • Synonyms
  • MUC1 & ERBB2; Mucin 1 & Erb-b2 receptor tyrosine kinase 2
  • Target II
  • HER2
  • Clone
  • Human
  • Target Species
  • ERBB2
  • Gene ID
  • 2064
Online Inquiry
Products

CONTACT US

USA: 45-1 Ramsey Road, Shirley, NY 11967, USA
Europe: Heidenkampsweg 58, 20097 Hamburg, Germany
Call us at:
USA: 1-631-381-2994
Europe: 44-207-097-1828
Fax: 1-631-207-8356
Email:

Online Inquiry

For any technical issues or products/services related questions, please leave your information below. Our team will contact you soon.

  • Verification code
    Click image to refresh the verification code.

Key Updates

Receive our latest news and insights.