All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
The vector of anti-CD47 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human CD47. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-CD47 antibody linked to CD28 and CD3ζ signaling domains. And the vector product was designed for the treatment of Ovarian cancer.
CAR Construction : huB6H12-CD28-CD3ζ Fig.1 Cytotoxicity assay in vitro. CD47-CAR-T cell showed cytotoxicity with CD47-positive cancer cell lines but not with CD47-negative Hela-CD19 cells. Golubovskaya, V., Berahovich, R., Zhou, H., Xu, S., Harto, H., Li, L., ... & Wu, L. (2017). CD47-CAR-T cells effectively kill target cancer cells and block pancreatic tumor growth. Cancers, 9(10), 139. |
CAR Construction : huB6H12-CD28-CD3ζ Fig.2 Determination of IL-2 release. Increased secretion of IL-2 by humanized CD47-CAR-T cells with CD47-positive cells SKOV-3 cells. IL-2 is not secreted by CD47-CAR-T cells with CD47-hegative cells, Hela-CD19 cells. Golubovskaya, V., Berahovich, R., Zhou, H., Xu, S., Harto, H., Li, L., ... & Wu, L. (2017). CD47-CAR-T cells effectively kill target cancer cells and block pancreatic tumor growth. Cancers, 9(10), 139. |
CAR Construction : Fig.1 Affinity determination by Surface Plasmon Resonance (SPR). Real time response curves of B6H12. Antibody concentrations were 200, 100, 50, 25, 12.5, 6.25 and 3.13 nM respectively. Zeng, D., Sun, Q., Chen, A., Fan, J., Yang, X., Xu, L., ... & Sun, Z. (2016). A fully human anti-CD47 blocking antibody with therapeutic potential for cancer. Oncotarget, 7(50), 83040. |
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