pCDCAR1 CD47 h(BBζ), T(CAR-T-2-L344-BZ)

Datasheet

The vector of anti-CD47 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human CD47. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-CD47 antibody linked to CD3-zeta and CD137 (4-1BB) signaling domains. And the vector product was designed for the treatment of B-cell malignancies.

Details

Target

CD47

Target Species

Human

Targeting Cell Type

T cell

Targeting Diseases

B-cell malignancies

Generation

Second

Vector Name

pCDCAR1

Vector Length

8kb

Vector Type

Lentiviral

Receptor Construction

scFv-41BB-CD3ζ

Discription of Signaling Cassetes

41BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Antibody Clone

B6H12.2

Antibody Host

Mouse

Target

Gene Name

CD47 molecule

Official Symbol

CD47

Gene ID

961

Synonyms

CD47; CD47 molecule; IAP; OA3; MER6; leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D8; CD47 antigen (Rh-related antigen, integrin-associated signal transducer)CD28; Chimeric antigen receptor; T cell; Retrovirus; CARs; Lentivirus; chimeric T cell receptors; chimeric immunoreceptors; chimeric antigen receptors; scFv; B6H12.2

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Related Products

Cat#	Description
VP-CAR-LC124	Lenti-CD47 CAR (scFv-CD28-FcεRIγ, B6H12.2) Viral Particle
CAR-T-3-L344-2BZ	Anti-CD47 scFv h(CD28-41BB-CD3ζ) CART, pCDCAR1
VP-CAR-LC125	Lenti-CD47 CAR (scFv-CD28-CD3ζ, B6H12.2) Viral Particle
VP-CAR-LC123	Lenti-CD47 CAR (scFv-CD3ζ, B6H12.2) Viral Particle
CAR-T-2-L344-2G	Anti-CD47 scFv h(CD28-FcεRIγ) CART, pCDCAR1
CAR-T-1-L344-2	Anti-CD47 scFv h(CD28) CART, pCDCAR1
CAR-T-3-L344-2XZ	Anti-CD47 scFv h(CD28-OX40-CD3ζ) CART, pCDCAR1
CAR-T-1-L344-G	Anti-CD47 scFv h(FcεRIγ) CART, pCDCAR1
CAR-T-1-L344-Z	Anti-CD47 scFv h(CD3ζ) CART, pCDCAR1
VP-CAR-LC127	Lenti-CD47 CAR (scFv-CD4-CD3ζ, B6H12.2) Viral Particle

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pCDCAR1 CD47 h(BBζ), T(CAR-T-2-L344-BZ)

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