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Anti-EGFR (Necitumumab) h(41BB-CD3ζ) CAR, pCDCAR1 (CAR-YF791)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-EGFR chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human EGFR. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-EGFR antibody linked to 41BB and CD3ζ signaling domains. And the vector product was designed for the treatment of NSCLC.

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Details

  • Target
  • EGFR
  • Targeting Cell Type
  • T Cell
  • Targeting Diseases
  • NSCLC
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-41BB-CD3ζ
  • Discription of Signaling Cassetes
  • 41BB
    CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.

    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • Necitumumab
  • Host
  • Human
  • Target Species
  • Human
  • Gene Name
  • Epidermal Growth Factor Receptor
  • Synonyms
  • EGFR;EGFR; Epidermal Growth Factor Receptor; Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian);

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  • Published Data
CAR scFv data FuncS

Fig.1 Antitumor capabilities of EGFR antibodies in EGFRvIII and wtEGFR-expressing gliomasphere lines.

CAR Construction : Latest CAR Construction

Fig.1 Antitumor capabilities of EGFR antibodies in EGFRvIII and wtEGFR-expressing gliomasphere lines.

Representative plots for the in vitro inhibition of proliferation in gliomaspheres expressing EGFRvIII after 7 days of treatment with 10 µg/mL of singular antibodies or domain III-targeting antibodies in combination with ch806 measured by Vialight assay

Jin, K. T., Chen, B., Liu, Y. Y., Lan, H., & Yan, J. P. (2021). Monoclonal antibodies and chimeric antigen receptor (CAR) T cells in the treatment of colorectal cancer. Cancer Cell International, 21(1), 1-15.

CAR scFv data FCM

Fig.2 EGFR expression on different human tumor cell lines.

CAR Construction : Latest CAR Construction

Fig.2 EGFR expression on different human tumor cell lines.

Analysis of cell surface EGFR expression level was performed by flow cytometry.

Albert, S., Arndt, C., Koristka, S., Berndt, N., Bergmann, R., Feldmann, A., ... & Bachmann, M. (2018). From mono-to bivalent: improving theranostic properties of target modules for redirection of UniCAR T cells against EGFR-expressing tumor cells in vitro and in vivo. Oncotarget, 9(39), 25597.

CAR scFv data FuncS

Fig.3 Comparison of the affinities of the mono- and bivalent EGFR-specific TMs.

CAR Construction : Latest CAR Construction

Fig.3 Comparison of the affinities of the mono- and bivalent EGFR-specific TMs.

For estimation of the respective Kd value increasing amounts of monovalent α-EGFR TM (triangle) or bivalent α-EGFR-EGFR TM (rectangle).

Albert, S., Arndt, C., Koristka, S., Berndt, N., Bergmann, R., Feldmann, A., ... & Bachmann, M. (2018). From mono-to bivalent: improving theranostic properties of target modules for redirection of UniCAR T cells against EGFR-expressing tumor cells in vitro and in vivo. Oncotarget, 9(39), 25597.

More Published Data More Published Data

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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