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pCDCAR1 ErbB2 h(4γ), NK (CAR-NK-2-M331-4G)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-ErbB2 (HER2) chimeric antigen receptor(CAR) is constructed for the engineering of NK cell to target human ErbB2. The NK cell is genetically modified through transduction with a lentiviral vector expressing scFv of anti-ErbB2 antibody linked to CD4 transmembrane domain and FcεRIγ signaling domains. And the vector product was designed for the treatment of breast carcinomas, ovarian carcinoma .

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Details

  • Target
  • ErbB2
  • Targeting Cell Type
  • NK cell
  • Targeting Diseases
  • Breast carcinomas; ovarian carcinoma
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • 8kb
  • Vector Type
  • Lentiviral
  • Receptor Construction
  • scFv-CD4-FcεRIγ
  • Discription of Signaling Cassetes
  • CD4
    Cluster of Differentiation 4 (CD4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). CD4 signaling in CAR ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation.
    FcεRIγ
    The high-affinity IgE receptor, also known as FcεRI, or Fc epsilon RI, is the high-affinity receptor for the Fc region of immunoglobulin E (IgE). FcεRI is a key molecule involved in allergic reactions. It is a tetramer composed of 1alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. The scFv-based CARs engineered to contain a signaling domain from FcεRIγ have been shown to deliver a potent signal for T cell activation and function.

Target

  • Clone
  • XHM298
  • Host
  • Rabbit
  • Target Species
  • Human
  • Gene Name
  • erb-b2 receptor tyrosine kinase 2
  • Synonyms
  • NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

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  • Published Data
Complete CAR data FuncS

Fig.1 Secretion of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) by CAR T cells assessed by cytometric bead array after 24 h exposure to synovial sarcoma cells.

CAR Construction : FRP5 scFv-41BB-CD28ζ Latest CAR Construction

Fig.1 Secretion of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) by CAR T cells assessed by cytometric bead array after 24 h exposure to synovial sarcoma cells.

HER2-targeted CAR T cells (blue bars) produced significantly higher levels of TNF-α and IFN-γ than T cells transduced with the empty vector (mock; orange bars). Data are presented as the mean ± standard deviation (SD) of triplicate experiments. The data are representative results from experiments using at least 2 healthy donors. ***P < 0.001.

Murayama, Y., Kawashima, H., Kubo, N., Shin, C., Kasahara, Y., Imamura, M., Oike, N., Ariizumi, T., Saitoh, A., Mihara, K., Umezu, H., Ogose, A., & Imai, C. (2021). Effectiveness of 4-1BB-costimulated HER2-targeted chimeric antigen receptor T cell therapy for synovial sarcoma. Translational oncology, 14(12), 101227.

More Published Data More Published Data

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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