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pCDCAR1 GD2 h(4ζ), T (CAR-T-2-M326-4Z)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-GD2 chimeric antigen receptor(CAR) is constructed for the engineering of T cells to target Human GD2. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-GD2 antibody linked to CD4 transmembrane domain and CD3-zeta signaling domains. And the vector product was designed for the treatment of neuroblastoma.

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Details

  • Target
  • GD2
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • Neuroblastoma
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • 8kb
  • Vector Type
  • Lentiviral
  • Receptor Construction
  • scFv-CD4-CD3ζ
  • Discription of Signaling Cassetes
  • CD4
    Cluster of Differentiation 4 (CD4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). CD4 signaling in CAR ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • ch14.18
  • Host
  • Chimeric
  • Target Species
  • Human
  • Gene Name
  • beta-1,4-N-acetyl-galactosaminyltransferase 1
  • Synonyms
  • GD2;B4GALNT1; beta-1,4-N-acetyl-galactosaminyltransferase 1; GALGT; SPG26; GALNACT; GalNAc-T;

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  • Published Data
Complete CAR data FuncS

Fig.1 GD2-CAR T cell killing assay.

CAR Construction : 14.18 scfv-CD28-41BB-CD3ζ Latest CAR Construction

Fig.1 GD2-CAR T cell killing assay.

Target cell killing analysis of the GD2 CAR T cells, 14.18. The OS cells transduced with a lentiviral wasabi green fluorescence reporter gene were used as target cells,
and plated into 96 or 48 wells for 2 hours before adding the different CAR T cells (Jurkat or primary T cells, at E:T ratio 3:1).

Chulanetra, M., Morchang, A., Sayour, E., Eldjerou, L., Milner, R., Lagmay, J., ... & Chang, L. J. (2020). GD2 chimeric antigen receptor modified T cells in synergy with sub-toxic level of doxorubicin targeting osteosarcomas. American journal of cancer research, 10(2), 674.

More Published Data More Published Data

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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