pCDCAR1 HER2 h(b2cζ), T(CAR-T-2-M550-CZ)
The vector of anti-HER2 chimeric antigen receptor(CAR) is constructed for the engineering of T cell to target human HER2. The T cell is genetically modified through transduction with a lentiviral vector expressing anti-HER2 VHH linked to b2c and CD3-zeta signaling domains. And the vector product was designed for the treatment of breast cancer, hepatocellular liver carcinoma.
Targeting Cell Type
Breast cancer; hepatocellular liver carcinoma
Discription of Signaling Cassetes
The b2c protein belongs to high-sulphur keratin family which comprises at least three proteins, B2A, B2B and B2C. And b2c in chimeric antigen receptors improve T-cell effector functions and enhance anti-tumor activity.
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
erb-b2 receptor tyrosine kinase 2
ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu; receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; CD28; Chimeric antigen receptor; T cell; Retrovirus; CARs; Lentivirus; chimeric T cell receptors; chimeric immunoreceptors; chimeric antigen receptors; scFv; OTI5C4
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
||Lenti-ErbB2 CAR (scFv-CD28-FcγR, N29) Viral Particle
||Human anti-ERBB2 (ErbB2 369–377) T Cell Receptor, pMSGV
||Anti-HER2 VHH h(CD8-CD3ζ) CAR, [T]
||Lenti-ErbB2 CAR (scFv-CD28-CD3ζ, OTI5C4) Viral Particle
||Lenti-HER2 CAR (scFv-CD28-41BB-CD3ζ, 7G) Viral Particle
||Anti-HER2 VHH h(CD28) CAR, [T]
||Lenti-ErbB2 CAR (scFv-CD28, OTI5C4) Viral Particle
||Lenti-ErbB2 CAR (scFv-b2c-CD3ζ, OTI5C4) Viral Particle
||Lenti-ErbB2 CAR (scFv-CD28-CD3ζ, C6-B1D2) Viral Particle
||Lenti-ErbB2 CAR (scFv-CD28-CD3ζ, 4D5 ) Viral Particle