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Anti-HMW-MAA scFv h(CD28) CART, pCDCAR1 (CAR-T-1-M318-2)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-HMW-MAA chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human HMW-MAA. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-HMW-MAA antibody linked to CD28 signaling domains. And the vector product was designed for the treatment of metastatic melanoma.

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Details

  • Target
  • HMW-MAA
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • Metastatic melanoma
  • Generation
  • First
  • Vector Name
  • pCDCAR1
  • Vector Length
  • 8kb
  • Vector Type
  • Lentiviral
  • Receptor Construction
  • scFv-CD28
  • Discription of Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatorysignals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells(APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.

Target

  • Clone
  • 225.28S
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • chondroitin sulfate proteoglycan 4
  • Synonyms
  • NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG

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  • Published Data
Complete CAR data ELISA

Fig.1 Cytokine IFN-γ release assay.

CAR Construction : 225.28S-CD28-CD3ζ Latest CAR Construction

Fig.1 Cytokine IFN-γ release assay.

Peripheral blood lymphocytes from two additional donors were transduced with the three reactive CARs and cocultured with CSPG4-positive cell lines from multiple different histologies. Each of the CSPG4 CAR transduced PBL cultures yielded IFN-γ effector cytokine release

Beard, R. E., Zheng, Z., Lagisetty, K. H., Burns, W. R., Tran, E., Hewitt, S. M., ... & Morgan, R. A. (2014). Multiple chimeric antigen receptors successfully target chondroitin sulfate proteoglycan 4 in several different cancer histologies and cancer stem cells. Journal for immunotherapy of cancer, 2(1), 1-11.

Complete CAR data FuncS

Fig.2 Specific killing by CSPG4 CAR transduced T cells.

CAR Construction : 225.28S-CD28-CD3ζ Latest CAR Construction

Fig.2 Specific killing by CSPG4 CAR transduced T cells.

CSPG4 CAR-transduced PBL were mixed with the indicated GSC lines at various effector to target ratios and cytolytic function determined.

Beard, R. E., Zheng, Z., Lagisetty, K. H., Burns, W. R., Tran, E., Hewitt, S. M., ... & Morgan, R. A. (2014). Multiple chimeric antigen receptors successfully target chondroitin sulfate proteoglycan 4 in several different cancer histologies and cancer stem cells. Journal for immunotherapy of cancer, 2(1), 1-11.

More Published Data More Published Data

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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